thiols and sulfur

Renal Transporters

What other transporters are working with NaS1 and SAT1?

NaS1 also known as slc13a1 is the sodium coupled sulfate transporter thatdepends n the electro chemical gradient of Na+ . SAT1 is a passive sulfate anion exchanger that is also known as Slc26a1. The goal was to figure out which part of the renal tubule these transporters are located and what other transporters are present.

This paper served as inspiration for a database study of Solute carrier (Slc) family members..

Lewis S, Chen L, Raghuram V, Khundmiri SJ, Chou CL, Yang CR, Knepper MA. “SLC-omics” of the kidney: solute transporters along the nephron. Am J Physiol Cell Physiol. 2021 Sep 1;321(3):C507-C518. PMC free article

This Slc transporter database lists mRNA trancripts and or proteins. There were region of the renal tubule. Only the parts of the renal tubule that also express slc26a1 are shown. Protein Levels (Thousands of Copies per Cell). There are over 100 Slc protein family members in the proximal tubule. Links to and are being provided where there is high expression. Additional notations are in bold cyan

Gene SymbolAnnotationSLC FamilyPTS1PTS2PTS3
Slc1a1excitatory amino acid transporter 3SLC101280
Slc1a4neutral amino acid transporter ASLC102755
Slc2a1facilitated glucose transporter member 1 (GLUT1)SLC20136127
Slc2a2facilitative glucose and fructose transporter (GLUT2)SLC2126110
Slc2a5fructose transporter (GLUT5)SLC2067224
Slc2a9urate transporterSLC20400
Slc2a13proton myo-inositol cotransporter, both come in.SLC20023
Slc3a1neutral and basic amino acid transport protein rBATSLC3522813963516
Slc3a24F2 cell-surface antigen heavy chainSLC3142671251
Slc4a4electrogenic sodium bicarbonate cotransporter 1 influc of both SLC458111810
Slc5a1sodium/glucose cotransporter 1SLC50432420
Slc5a2sodium/glucose cotransporter 2SLC52452531
Slc5a6sodium-dependent multivitamin transporterSLC5062489
Slc5a8sodium-coupled monocarboxylate transporter 1SLC508061148
Slc5a9sodium/glucose cotransporter 4SLC505455
Slc5a10sodium/glucose cotransporter 5SLC50288640
Slc5a11sodium/myo-inositol cotransporter 2SLC507499
Slc5a12sodium-coupled monocarboxylate transporter 2SLC54600
Slc6a6sodium- and chloride-dependent taurine transporterSLC60032
Slc6a8sodium- and chloride-dependent creatine transporter 1SLC615219928
Slc6a13sodium- and chloride-dependent GABA transporter 2SLC60351
Slc6a18sodium-dependent neutral amino acid transporter B(0)AT3SLC605851059
Slc6a19sodium-dependent neutral amino acid transporter B(0)AT1SLC6070
Slc6a20sodium- and chloride-dependent transporter XTRP3SLC606645
Slc7a7Y+L amino acid transporter 1SLC722850
Slc7a8large neutral amino acids transporter small subunit 2SLC7369820
Slc7a13solute carrier family 7 member 13SLC701847510
Slc9a1sodium/hydrogen exchanger 1SLC90130
Slc9a3sodium/hydrogen exchanger 3SLC9111060
Slc10a1sodium/bile acid cotransporterSLC100130
Slc10a2ileal sodium/bile acid cotransporterSLC100390
Slc12a1Na-K-2Cl cotransporter 2, bumetanide sensitive (NKCC2)SLC12040
Slc12a6K-Cl cotransporter 3SLC122275
Slc12a7K-Cl cotransporter 4SLC1224381
Slc12a9putative cation-chloride cotransporter-interacting protein 1SLC120161
Slc13a1sodium/sulfate cotransporter 1SLC13110
Slc13a2sodium-dependent dicarboxylate transporter 1SLC13068127
Slc13a3sodium-dependent dicarboxylate transporter 2SLC1310015432
Slc15a1oligopeptide transporterSLC15050
Slc15a2kidney H(+)/peptide cotransporterSLC1503811591
Slc15a4peptide/histidine transporter 1 (PHT1)SLC15070
Slc16a1monocarboxylate transporter 1SLC1612591900
SLC16a2monocarboxylate transporter 8SLC16010
Slc16a4monocarboxylate transporter 5SLC160184124
Slc16a10monocarboxylate transporter 10SLC166617161
Slc16a11monocarboxylate transporter 11SLC160028
Slc16a12monocarboxylate transporter 12SLC1675670
Slc16a13monocarboxylate transporter 13SLC160500
Slc17a3sodium-dependent phosphate transport protein 4SLC1701890
Slc19a1reduced folate transporterSLC1903062
Slc19a3thiamine transporter 2SLC19002
Slc22a1organic cation transporter 1 (mOCT1)SLC22881502
Slc22a12urate anion exchanger 1SLC220528419
Slc22a13organic cation transporter-like 3 (ORCTL-3)SLC2201163
Slc22a2organic cation transporter 2 (mOCT2)SLC22120044652
Slc22a22prostaglandin-specific organic anion transporterSLC22041295
Slc22a25solute carrier family 22 member 25SLC2206040
Slc22a4organic cation/carnitine transporter 1SLC220223
Slc22a5organic cation/carnitine transporter 2SLC22323657106
Slc22a6renal organic anion transporter 1 (mROAT1)SLC227020970
Slc22a7organic anion transporter 2 (mOAT2)SLC220199
Slc22a8organic anion transporter 3 (mOAT3)SLC222412560
Slc23a1Na(+)/L-ascorbic acid transporter 1SLC234811562375
Slc23a3Na(+)/L-ascorbic acid transporter 3SLC23021188
Mtch1mitochondrial carrier homolog 1SLC250350
Slc25a1tricarboxylate transport protein, mitochondrialSLC2541523208690
Slc25a10mitochondrial dicarboxylate carrierSLC25122663873183
Slc25a11mitochondrial 2-oxoglutarate/malate carrier proteinSLC2582334854113
Slc25a12calcium-binding mitochondrial carrier protein Aralar1SLC2589531254
Slc25a13calcium-binding mitochondrial carrier protein Aralar2SLC2547636192531
Slc25a15mitochondrial ornithine transporter 1SLC25159423173
Slc25a20mitochondrial carnitine/acylcarnitine carrier proteinSLC255141593714
Slc25a21mitochondrial 2-oxodicarboxylate carrierSLC25016133
Slc25a24calcium-binding mitochondrial carrier protein SCaMC-1SLC25060
Slc25a3phosphate carrier protein, mitochondrialSLC2511474108335227
Slc25a30kidney mitochondrial carrier protein 1SLC2502174
Slc25a35solute carrier family 25 member 35SLC257534
Slc25a36solute carrier family 25 member 36SLC250580
Slc25a4ADP/ATP translocase 1SLC253312751433
Slc25a40solute carrier family 25 member 40SLC251200
Slc25a42mitochondrial coenzyme A transporter SLC25A42SLC2519255
Slc25a44solute carrier family 25 member 44SLC250210
Slc25a45solute carrier family 25 member 45SLC25482480
Slc25a46solute carrier family 25 member 46SLC25024102
Slc25a48solute carrier family 25 member 48SLC250421
Slc25a5ADP/ATP translocase 2, mitochondria X chromosomeSLC25240332625428217
Slc25a51mitochondrial nicotinamide adenine dinucleotide transporterSLC25914661
Slc26a1sulfate anion transporter 1SLC26893600
Slc27a2very long-chain acyl-CoA synthetaseSLC27109666482
Slc27a4long-chain fatty acid transport protein 4SLC270110
Slc28a1sodium/nucleoside cotransporter 1SLC2870960
Slc29a1equilibrative nucleoside transporter 1SLC290550
Slc29a3equilibrative nucleoside transporter 3SLC291077591
Slc30a1zinc transporter 1SLC30042186
Slc30a5zinc transporter 5SLC30007
Slc30a9zinc transporter 9SLC30152628
Slc31a2probable low affinity copper uptake protein 2 Copt1 Cu transport within cell SLC310670
Slc33a1acetyl-coenzyme A transporter 1SLC3301947
Slc34a1sodium-dependent phosphate transport protein 2ASLC345024591132
Slc34a3sodium-dependent phosphate transport protein 2CSLC3410800
Slc35a1CMP-sialic acid transporterSLC352711
Slc35b2adenosine 3′-phospho 5′-phosphosulfate transporter 1SLC3521140
Slc35e1solute carrier family 35 member E1SLC350182
Slc35f2solute carrier family 35 member F2SLC3508682
Slc37a4glucose-6-phosphate exchanger SLC37A4SLC37585530
Slc38a3sodium-coupled neutral amino acid transporter 3SLC380159203
Slc39a7zinc transporter SLC39A7SLC39144109108
Rhcgammonium transporter Rh type CSLC420047
Slc43a2large neutral amino acids transporter small subunit 4SLC4327610
Slc44a4choline transporter-like protein 4SLC4406596
Slc47a1multidrug and toxin extrusion protein 1SLC4711414862229
Flvcr2feline leukemia virus subgroup C receptor-related protein 2SLC4900282
Slc49a3major facilitator superfamily domain-containing protein 7ASLC490173
Slc52a3riboflavin transporter 2 (RFT2)SLC520239154
Mpc1mitochondrial pyruvate carrier 1SLC5412739601071
Mpc2mitochondrial pyruvate carrier 2SLC54591159371
Letm1mitochondrial proton/calcium exchanger proteinSLC5520910161408
Magt1magnesium transporter protein 1SLC58137242223
Mfsd4bmajor facilitator superfamily domain-containing protein 2BSLC601324340
Spns1protein spinster homolog 1SLC63034130
Tmem165transmembrane protein 165SLC640331
Slco4c1solute carrier organic anion transporter family member 4C1SLCO075394
from an nbci database on slc proteins

Just an acknowledgement that the protein levels do not always match the mRNA levels. Slc13a1/NaST1 was not in these stables from the Lewis publication. We do see that Slc26a1 (arrows) is restricted to proximal tubule segments PTS1-2.

This exploration inspired by the Lewis publication has make it so very clear that our kidneys are very busy and require very large amounts of energy. Note that many of the Slc family members in the proximal tubule are mitochondria proteins.

Leave a Reply