clays and binders, microbiomes

Phillipsite and microbiome

This post started out as a post on clays and humic/fulvic acid. Zeolites are common clays mixed with

Superchi P, Saleri R , Ossiprandi MC et al (2017) Natural zeolite (chabazite/phillipsite) dietary supplementation influences faecal microbiota and oxidant status of working dogs. Italian Journal of Animal Science Volume 16, 2017 – Issue 1 free article

These dogs were eating an almost human diet and had the same variability in age and weight as one would expect for humans. The dogs were considered healthy, based on physical examination and blood biochemistry analysis and did not receive medications that are expected to alter the gut microbiota (i.e. antibiotics). The dogs were also dewormed.

  • Forty English Setter dogs reared in the same kennel were involved in the study with two replicates separated by time (20 dogs/replicate).
  • Inside each replication, the animals were subjected to the same environmental conditions.
  • Mean age (years) was 3.46 ± 2.1
  • mean body weight (BW, kg) was 19.02 ± 3.2.
  • At each replicate, dogs were divided into two homogeneous groups (10 dogs/group), based on their age and weight. In both groups, sexes (5 males, 5 not pregnant females) were equally distributed.
  • The rather varied diet consisted of 25 g dry matter/kg of BW0.75, once a day, at 17:00 h,
  • supplemented (Z group) or not supplemented (C group) with chabazite/phillipsite at the dose of 5 g/head/day.
a Providing per kilogram of diet: vitamin A, 12.500 UI; vitamin D3, 400 UI; vitamin E, 50 mg; vitamin B1, 2 mg; vitamin B2, 4 mg; vitamin B6, 2 mg; vitamin B12, 0.010 mg; vitamin C, 100 mg; vitamin PP, 20 mg; vitamin K, 2 mg; Biotin, 0.2 mg; Fe, 800 mg; Cu, 15 mg; Mn, 35 mg; Se, 0.05 mg; Zn, 110 mg.

This philliposite has a lot of moisture. How does the moisture effect the performance? The iron content could also be interesting. Chabazite has a fromula Ca,K2,Na2,Mg)Al2Si4O12•6H2O

Note that in addition to consuming the commercial extruded mixed feed, aka kibbles, the dogs were eating bread and chicken. The

Table 3 a C = untreated group with chabazite/phillipsite, Z = treated group with chabazite/phillipsite;.
b SEM, standard error of the difference of means.
c On a five points (1–5) scale.
d Samples collected post trial.
e,f Different letters in the same row indicate statistical difference (p < .05).

Table 4 a C: untreated group with chabazite/phillipsite; Z: treated group with chabazite/phillipsite.

b SEM: standard error of the difference of means.

c,d Different letters in the same row indicate statistical difference (p < .05).

The statistics used in this publication are confusing. The say they used a GLM, or general linear model, and ANOVA.


Thiobarbituric acid reactive substances (TBARS) are formed as a byproduct of lipid peroxidation usually malondialdehyde,

The origin of thiobarbituric acid (TBAA) reactive substances and the reactant used for quantitation.

The amazing part of this study is that TBARS went down, even though there was iron in the philliposite clay. The presence of iron and hydrogen peroxide, H2O2, and generate hydroxyl radicals .OH in a process known as the Fenton Reaction. and Haber-Wess Reactions

The presence of Fe and


An interesting aspect of this report is that the authors used low cost agar plates to estimate changes in the microbiome. The family groups they looked at contain some good and bad members.

screen captures from Wikipedia explaining phylogeny of bacteria in the commercially available agar plates..

As low budget as some this study is, the results are kind of exciting. This comment really is meant as praise.

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